Inhibitory Effect of Luteolin Liposome Solution by Animal Model for Atopic Dermatitis in NC/Nga Mice / 한국실험동물학회지

Atopic dermatitis is a common chronic inflammatory skin disease, associated with marked inflammatory cells (of mast cells and eosinophils) and severe itching, which leads to clinical problems in the pediatric population. This study was designed to investigate the inhibitory effects of luteolin liposome solution, that is entrapped the hydrophobic luteolin (one of the flavonoids) into ethosome to improve its stability, by using hapten-induced atopic dermatitis animal model (NC/Nga mice).The luteolin liposome treated mice showed anti-inflammatory effect as evidenced by the lowering of erythema and edema in clinical observation, reduction of inflammatory cell infiltration and epidermal thickness in histopathological examination, when compared with TNCB induced controls. Luteolin liposome solution also reduced the serum IgE level which played important roles in the atopic dermatitis model. These results suggest that luteolin liposome solution has some merit in this formulation showing inhibitory effects for the atopic dermatitis.

Anti-adhesive Effect of Poloxamer/Alginate/CaCl2 Mixture in the Rat Model

PURPOSE: The occurrence of post-surgical adhesion is still a major cause of postoperative morbidity due to the lack of satisfactory treatment or prophylaxis. Several adhesion barriers have been developed in the form of solutions or membrane in an attempt to solve these problems. However both types of tissue barriers have some limitations in their practical applications. In order to overcome these problems, a temperature-sensitive Poloxamer/Alginate/CaCl2 mixture was prepared as an adhesion barrier. With this material, toxicity, inflammation and the adhesion prevention effect was evaluated in an animal model. METHODS: The sol-gel transition behavior was measured using a viscometer. An in vitro gel stability test and an in vivo degradation test was performed. The anti-adhesion effect was evaluated using a cecal-abdominal wall abrasion model. The denuded cecum was coated with Poloxamer/ Alginate/CaCl2 mixture, GUARDIX-SL (positive control group) or neither (negative control group) and apposed to the abdominal wall (each n=14). One week after surgery, the level of adhesion was graded from zero to three using a whole-number system. RESULTS: The LCST of the poloxamer/sodium alginate mixture was 25 degrees C. The gel stability of Poloxamer was improved by adding mild cross-linked sodium Alginate/CaCl2 mixture. The adhesion grade and area were significantly lower in the experimental group than in the control. CONCLUSION: The anti-adhesive effect of the Poloxamer/Alginate/CaCl2 mixture was comparable to the previously- developed solution type barrier and all the materials had degraded within 21 days. From these results, Poloxamer/ Alginate/CaCl2 mixture is a good candidate for use as a coatable or injectable tissue adhesion barrier.

The Pre-test for Development of Insole which Maintains Constant Temperature in Shoe

OBJECTIVE: To investigate the change in plantar skin temperature in normal subjects wearing five different kinds of insoles. METHOD: Twenty normal subjects were chosen and were made to walk on a treadmill for five 30-minute sessions wearing the same shoes, and wearing five different kinds of insoles (four types were endothermic insoles, but one type was a normal insole) respectively. Subjects sat on a chair for 10 minutes before and after walking. The plantar skin temperature was recorded on 4 sites (from channel 1 to 4) on each left insole. The plantar skin temperature was recorded every 10 seconds for a total of 50 minutes, with T-type Thermocouple(R). RESULTS: There were no statistical differences in the change of plantar skin temperature for each insole before, during and after walking. No statistical differences in subjective thermal sense and Borg Rating on perceived exertion scale for each insole before, during and after walking. Time in peak plantar temperature was 25.9~28.3 minutes (after subjects starts walking), wearing normal insole. CONCLUSION: There was no statistical difference in the change of plantar skin temperature for the five different kinds of insoles However, we found this method to be effective in examining plantar skin temperature during walking.

GUARDIX-SL(R) in Sinus Surgery: A Preclinical Animal Study / 대한이비인후과학회지

BACKGROUND AND OBJECTIVES: The most frequent complication of FESS (functional endoscopic sinus surgery) is stenosis of the operation site. The complication may influence the postoperative wound healing and the outcome of surgery. There are several measures proposed to prevent the complication. We sought to determine the efficacy of GUARDIX-SL which is made from sodium hyaluronate and sodium carboxymethylcellulose in soluble form. MATERIALS AND METHOD: For 7 rabbits, we made a surgical opening anterior to sinus ostium of the maxillary sinuses on both sides. We put the gelfoams soaked with GUARDIX-SL into the left openings and those soaked with normal saline into the right openings as a control group. We measured the size of the openings after two weeks. RESULTS: There was no synechiae in GUARDIX-SL group whereas we found synechiae developed in two openings in the control group. The mean area of the opening was 9.29 mm2 and 1.61 mm2 in GUARDIX-SL group and the control group (p=0.027), respectively. CONCLUSION: GUARDIX-SL was effective in reducing postoperative stenosis in the animal model.

Evaluation of Resorbable Materials for Preventing Surgical Adhesion on Rat Experiment

PURPOSE: Postsurgical adhesion formation is still a cause of postoperative morbidity because no satisfactory treatment or prophylaxis has yet been developed. Therefore, we have established an animal model for adhesion prevention, and evaluated the toxicity, inflammation and adhesion prevention effect of available water soluble materials as barriers against adhesion formation. METHODS: Four-week old female Sprague-Dawley rats (Sam: TacN(SD)BR, Smatako Co., Seoul Korea) were used. The 2 cm2 of cecal serosa and adjacent abdominal wall were abraded with bone burr, and the serosa of the cecum was sutured to the abdominal wall 1 cm apart from the injured site. The denuded cecum was covered with either sodium hyaluronate (HA), sodium alginate (Alg), sodium carboxymethyl cellulose (CMC), polyethylene glycol-polypropylene glycol-polyethylene glycol (PEG-PPG, Poloxamer), or polyethylene glycol (PEG) on both sides, or neither side (control group) and apposed to the abdominal wall. Two weeks after operation, the quality of adhesion was graded using a whole-number system of zero to five. RESULTS: Adhesion grades were highest in the control group (average 4.8), were a little lower in the PEG-PPG (aver. 4.6) and PEG (aver. 4.1) groups, and were significantly lower in the HA (aver. 3.1), Alg (aver. 3.3) and CMC (aver. 3.0) groups (all, P<0.05). However but inflammation was revealed in the Alg and CMC groups. CONCLUSION: In our animal model for adhesion prevention, a fairly good antiadhesion effect was attained with Alg, CMC and HA, whereas inflammation was revealed with Alg and CMC. In conclusion, HA is the best candidate for adhesion prevention.